Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins

Figure 3

PBMN-derived neural aggregates exhibit low FOXG1 expression levels. (a) Dot plots and box plots showing FOXG1 and OTX1 expression in day 14 neural aggregates cultured in the various conditions. (b) Dot plots and box plots showing the FOXG1 expression levels according to tissue origin. The number of clones analyzed is indicated in parentheses. Statistical analysis was performed by the Steel-Dwass test.. (c) Clustering of clones, based on the expression of FOXG1 and SOX1, using the -medoids method. The delta Ct values are indicated by “+” symbols. Four clusters were identified: FOXG1-high/SOX1-high (red), FOXG1-middle/SOX1-high (green), FOXG1-low/SOX1-high (black), and FOXG1-low/SOX1-low (pink). See also Table S3.

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