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Stem Cells International
Volume 2016, Article ID 7235757, 10 pages
http://dx.doi.org/10.1155/2016/7235757
Research Article

Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins

1Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka 540-0006, Japan
2Division of Stem Cell Research, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka 540-0006, Japan
3Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan
4Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan
5Department of Orthopedic Surgery, Keio University School of Medicine, Tokyo 160-8582, Japan
6Department of Neurosurgery, Osaka National Hospital, National Hospital Organization, Osaka 540-0006, Japan

Received 24 December 2015; Accepted 28 March 2016

Academic Editor: Kaylene Young

Copyright © 2016 Hayato Fukusumi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplementary material contains three figures (Figure S1, S2, and S3) and four tables (Table S1, S2, S3, and S4). Figures S1, S2, and S3, which are related to Figure 1 and 2, show the morphologies and delta Ct values for gene expression in hiPSCs, EBFM-derived EBs, and dSMADi-derived neural aggregates. Table S1 shows the information regarding the hiPSCs used in this study. Table S2, which is related to Figure 2, compares the effects of two types of culture media and oxygen levels on the size of day 7 and day 14 neural aggregates. Table S3, which is related to Figure 3, shows the list of neural aggregates clustered based on FOXG1 and SOX1 expression levels. Table S4 presents the primer sequences used for quantitative RT-PCR.

  1. Supplementary Material