Periostin: A Downstream Mediator of EphB4-Induced Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
Evaluation of osteogenic differentiation after treatment with ephrinB2-FC or POSTN. (a) ALP staining was performed after 9 days in culture in osteogenic medium with stimulation. Untreated MSCs were used as the control. (b) The sum integral optical density (IOD) was quantified using Image-Pro Plus 6.0. Bars represent means ± SD from 3 biological replicates; . (c) Alizarin red S staining was done after 21 days in culture in the same conditions. (d) The sum IOD again was quantified by Image-Pro Plus 6.0. Bars represent means ± SD from 3 biological replicates; .
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