The Engrailed-1 Gene Stimulates Brown Adipogenesis

Figure 2

Expressions of EN1 in BAT at pathophysiological conditions. (a, c, and e) mRNA expression and (b, d, and f) protein expressions of EN1 in BAT of mice in different pathophysiological conditions: room temperature (RT) and 4°C for 8 hours (cold); 3-week-old male C57BL/6J mice fed low-fat diet (LFD) or high-fat diet (HFD) for 8 weeks; 8-week-old C57BL/6J (WT) and Ob/Ob mice were analyzed. Relative levels of EN1 protein were calculated based on densitometry analysis (bottom panel of (b), (d), and (f)). Data are mean ± SEM. , , .

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