Research Article
The Engrailed-1 Gene Stimulates Brown Adipogenesis
Figure 4
Overexpression of EN1 stimulates brown adipogenesis. Lentiviral mediated overexpression of EN1 was confirmed by (a) RT-PCR or (b) western blot analysis. Brown adipogenesis was assessed by (c) phase contrast microscopy and Oil-Red O staining. (d, e) Adipogenic and thermogenic gene expression and (f) BAT related protein expressions were analyzed during brown adipogenesis. Data are mean ± SEM. , , .
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |