Epigallocatechin-3-gallate Protects against Hydrogen Peroxide-Induced Inhibition of Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells

Figure 1

Effects of H₂O₂ and EGCG on cell viability of human BM-MSCs. (a) Characterization of human BM-MSCs by flow cytometry. The majority of the cells are CD29+, CD44+, CD105+, and CD34−, which are typical characteristic phenotypes of BM-MSCs. Effects of different concentrations of H₂O₂ exposure (0.1 to 2 mM) (b) or EGCG exposure (5 to 100 μM) (c) for 24 h on cell viability of BM-MSCs, measured by MTT assay. (d) Cotreatment of 0.2 mM H₂O₂ and 5 μM EGCG for indicated time on cell viability of BM-MSCs. Data were presented as mean ± SEM. versus control and versus 0.2 mM H₂O₂ treatment alone.

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