Epigallocatechin-3-gallate Protects against Hydrogen Peroxide-Induced Inhibition of Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells

Figure 4

5 μM EGCG rescues the inhibition of osteogenic differentiation induced by 0.2 mM H₂O₂ involved in the Wnt pathway. (a) Western blot analysis of Wnt pathway-related regulators β-catenin and cyclin D1 in the cultures of experimental groups. (b) Relative protein expressions normalized to GAPDH in (a). Wnt inhibitor DKK-1 blocked the protective effects of EGCG on the inhibition of osteogenic differentiation by 0.2 mM H₂O₂ exposure, as evidenced by ALP activity (c), calcium contents (d), and relative mRNAs expression of Runx2 and OSX (e). Data were presented as mean ± SEM. and versus control, versus 0.2 mM H₂O₂ treatment alone in (b), and versus cotreatment of 0.2 mM H₂O₂ and 0.1 μM EGCG.

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