Effect on Multipotency and Phenotypic Transition of Unrestricted Somatic Stem Cells from Human Umbilical Cord Blood after Treatment with Epigenetic Agents

Figure 5

Telomere length and expression and DNA methylation of hTERT in SpheUSSCs. (a) Shows telomeres length of SpheUSSCs, USSC8/25, and ESC-H9, as detected by telomerase assay. (b) hTERT transcription analysed by conventional PCR in the ES cell line H9 and in SpheUSSCs is shown. DNA methylation patterns of the hTERT 5′ region of USSCs and SpheUSSCs as uncovered by NimbleGen analysis are shown in (c). The higher a bar is, the higher the DNA methylation is at the corresponding 75 bp long CpG rich DNA sequence.