Human Very Small Embryonic-Like Stem Cells Are Present in Normal Peripheral Blood of Young, Middle-Aged, and Aged Subjects

Figure 1

FCM analysis of PB-derived VSELs. Erythrocytes were removed by hypotonic lysis and PB NCs were stained with fluorescein isothiocyanate-lineage markers (Lin), phycoerythrin-CD45 and allophycocyanin-CD133, CD34, or CXCR4 mAbs. (a) Left dot plot: 10⁶ events ranging from 2 to 10 µm were included in the P2 gate. Right histogram: Lin⁻ cells were included in the P1 analysis gate. Middle histogram: P1 gated cells stained with 7-aminoactinomycin D (7-AAD) were included in the P2 gate. Right histogram: Lin⁻ cells were included in the P3 gate and were analyzed for CD45 coexpression with CD133, CD34, or CXCR4 antigens. (b) Dot plots showing the size of analyzed CD133⁺, CD34⁺, or CXCR4⁺, Lin⁻, CD45⁻ VSELs (upper row) and CD133⁺, CD34⁺, or CXCR4⁺, Lin⁻, CD45⁺ HSC (lower row).

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