Human Bone Marrow Stromal Cells: A Reliable, Challenging Tool for In Vitro Osteogenesis and Bone Tissue Engineering Approaches

Table 1

Primer pairs used for real time PCR.

Gene	Accession number	Forward primer	Reverse primer	Binding position	Product length (bp)	Product identity (%)^##
actin	NM001101	aatgtggccgaggactttgattgc	ttaggatggcaagggacttcctgt	1414–1508	95	100
oct-4	NM203289	cttcaggagatatgcaaa	ccggttacagaacca	1367–1561	195	93
cbfa1	NM00102463	aatgacaccaccaggccaat	tggcctacaaaggtgggttt	3646–3805	160	100
pthr	NM000316	ttggcgtccactacattgtc	tccctggaaggagttgaaga	1453–1559	107	98
bglap	NM199173	ggtggagcctttgtgtccaagc	gtcagccaactcgtcacagtcc	169–327	159	97
ppar	NM138711	tccgtggatctctccgtaat	ctgcaaccactggatctgtt	302–437	136	96

Genes: actin,  -actin; oct-4, octamer binding transcription factor 4; cbfa, core-binding factor subunit alpha-1; pthr, parathyroid hormone receptor; bglap, bone gla protein = osteocalcin; ppar, peroxisome proliferator-activated receptor gamma.
Determined by sequencing of amplified DNA products.