Human Bone Marrow Stromal Cells: A Reliable, Challenging Tool for In Vitro Osteogenesis and Bone Tissue Engineering Approaches
Table 2
Donor data (number, age, BMI, and TNAP activity) and correlation data.
Age groups/yrs.
All donors
Females
Males
18–68
18–25
26–35
>35
18–68
18–25
26–35
>35
18–68
18–25
26–35
>35
Number of donors
Entire cohort
531
169
187
175
168
49
59
60
363
120
128
115
Age of donors
Mean ± SEM/yrs.
32.0 ± 0.39
22.6 ± 0.14
30.2 ± 0.20
43.1 ± 0.39
32.5 ± 0.74
22.4 ± 0.27
29.6 ± 0.35
43.5 ± 0.75
31.8 ± 0.46
22.7 ± 0.16
30.5 ± 0.24
42.8 ± 0.45
TNAP activity in BM
Mean ± SEM/mU/mg protein
176 ± 8.2
156 ± 13.8
175 ± 14.4
196 ± 14.1
215 ± 17.4
196 ± 31.9
228 ± 31.7
217 ± 27.1
158 ± 8.7
139 ± 14.4
150 ± 14.8
185 ± 16.2
TNAP activity in OM/D
Mean ± SEM/mU/mg protein
470 ± 19.6
535 ± 39.4
446 ± 34.5
434 ± 27.1
460 ± 35.2
532 ± 67.0
482 ± 74.4
380 ± 36.1
475 ± 23.7
536 ± 48.4
429 ± 37.0
462 ± 36.5
TNAP inducibility
Mean ± SEM
3.97 ± 0.16
4.68 ± 0.28
3.87 ± 0.27
3.39 ± 0.27
3.01 ± 0.25
3.28 ± 0.31
2.71 ± 0.37
3.09 ± 0.53
4.41 ± 0.20
5.25 ± 0.37
4.40 ± 0.35
3.55 ± 0.30
TNAP activity in BM versus inducibility
-square
0.1456
0.0997
0.1584
Frequency versus inducibility (Gaussian fit)
-square
0.7055
0.337
0.4116
0.3484
0.3629
0.5709
Mean
2.5
3.46
2.15
2.02
1.951
2.74
SD ()
1.83
2.54
2.15
1.87
1.86
2.29
68.3% range
0.67–4.34
0.91–6.0
0–4.3
0.14–3.89
0.1–3.81
0.45–5.03
50.0% range
1.26–3.74
1.74–5.17
0.69–3.6
0.75–3.28
0.7–3.20
1.28–4.29
TNAP in BM versus age
value
0.7729
0.5611
0.3741
0.2692
0.5058
0.0726
0.3910
0.4781
-square
0.0164
0.0005
0.0018
0.0618
0.0048
0.0259
0.0078
0.0545
0.0266
0.0662
0.0040
0.0641
TNAP in OM/D versus age
value
0.0450
0.2871
0.9025
0.3541
0.2579
0.5023
0.1167
0.2736
0.6400
0.7921
0.9956
0.0638
-square
0.0230
0.0021
0.0001
0.0046
0.0077
0.0096
0.0426
0.0206
0.0006
0.0006
0.0001
0.0301
Age versus inducibility
value
0.3024
0.3811
0.0748
0.5715
0.1116
0.6463
0.2421
0.7497
0.2091
0.2224
-square
0.0227
0.0064
0.0041
0.0182
0.0020
0.0530
0.0037
0.0024
0.0368
0.0009
0.0125
0.0132
Number of donors
With BMI data
338
105
233
BMI
Mean ± SEM
25.2 ± 0.20
24.1 ± 0.40
25.6 ± 0.23
Age versus BMI
value
0.0802
-square
0.0219
0.0294
0.0264
TNAP in BM versus BMI
value
0.4832
0.5138
0.4266
-square
0.0014
0.0042
0.0027
TNAP in OM/D versus BMI
value
0.8312
0.5478
0.6799
-square
0.0001
0.0035
0.0007
Inducibility versus BMI
value
0.4232
0.7769
0.8808
-square
0.0019
0.0008
0.0001
TNAP, tissue nonspecific alkaline phosphatase; BM, basic medium; OM/D, osteogenic differentiation medium; inducibility calculated OMD : BM values from each donor separately; BMI, body mass index [weight [kg]/height [m]2]. Significant correlation; for further statistical analyses see figures and text.
