Hypoxia Inducible Factor-1α Regulates the Migration of Bone Marrow Mesenchymal Stem Cells via Integrin α₄

Figure 3

Alteration of integrin -mediated signaling pathway in BM-MSCs under hypoxia. The mRNA expression levels of HIF-1α (a) and integrin (b) in BM-MSCs were determined by real-time PCR. 18S rRNA was used as the loading control. Protein expression levels of integrin (c) and ROCK1 and Rac1/2/3 (d) in BM-MSCs were assessed by Western blotting. GAPDH was used as the loading control. (compared with YC-1 nontreated group) and (compared with normoxic group). HIF-1α: hypoxia-inducible factor-1α; ITGA4: integrin ; ROCK1: Rho-associated kinase 1.

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