Research Article
Hypoxia Inducible Factor-1α Regulates the Migration of Bone Marrow Mesenchymal Stem Cells via Integrin α4
Figure 4
The effect of integrin inhibition on BM-MSC migration and activities of MMPs under hypoxia. (a) The mRNA expression of integrin in BM-MSCs was suppressed by transfection of integrin siRNA. 18S rRNA was used as the loading control. (b) BM-MSC migration was significantly increased after siITGA4 transfection. Invasiveness of BM-MSCs was assessed by invasion assay (left). BM-MSCs invaded through the inserts were counted for quantification (right). (c) Enzymatic activities of MMP-9 and MMP-2 in BM-MSCs after siITGA4 transfection were determined by zymography (left). Quantification of enzymatic activities of MMP-9 (middle) and MMP-2 (right). (compared with siITGA4 nontransfected group) and (compared with normoxic group). MMP: matrix metalloproteinase; siITGA4: integrin α4 siRNA.
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