Research Article
Hypoxia Inducible Factor-1α Regulates the Migration of Bone Marrow Mesenchymal Stem Cells via Integrin α4
Figure 5
Interaction between integrin and HIF-1α and its effect on expression of Rho GTPases under hypoxia. (a) Protein expression levels of integrin , HIF-1α, ROCK1, and Rac1/2/3 were assessed by Western blotting. GAPDH was used as the loading control. (b) HIF-1α and integrin were localized with immunofluorescence in BM-MSCs after siITGA4 transfection under normoxic or hypoxic conditions. Blue: DAPI; green: HIF-1α; red: integrin . Scale bar = 80 μm (400x original magnification). (compared with siITGA4 nontransfected group) and (compared with normoxic group). DAPI: 4′,6-diamidino-2-phenylindole; HIF-1α: hypoxia-inducible factor-1α; ITGA4: integrin ; ROCK1: Rho-associated kinase 1; siITGA4: integrin siRNA.
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