Role of MSX1 in Osteogenic Differentiation of Human Dental Pulp Stem Cells

Figure 2

Effects of MSX1 knockdown on calcification of hDPSCs. hDPSCs were transfected with either MSX1 siRNA (s8999 or s224066) or control siRNA and incubated for 2 days in growth medium before the cultures became confluent. Thereafter, the cultures were exposed to osteogenesis-induction medium. (a) The calcified matrix was stained with alizarin red on day 14. (b) MSX1 mRNA level was quantified by RT-qPCR at 48 h after transfection. Values are averages ±SD for three cultures. .

(a)

(b)