Role of MSX1 in Osteogenic Differentiation of Human Dental Pulp Stem Cells

Figure 5

Effects of MSX1 knockdown on the expression of cholesterol synthesis-related genes, which are direct targets for SREBP2, in hDPSCs. (a) The cholesterol biosynthesis pathway is shown and the bold frames indicate target genes for the master transcriptional factor SREBP2. Microarray analysis indicates all genes involved in cholesterol synthesis are upregulated by MSX1 knockdown. The numbers in brackets represent the fold changes in gene expression in MSX1-knockdown cells as compared with the control cells. (b) The mRNA levels of genes relevant to cholesterol synthesis, including SREBP2, HMGCS1, HMGCR, FDPS, CYP51A1, and DHCR7, were quantified by RT-qPCR analysis. Values are averages ±SD for three cultures. ; .

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