Research Article
Role of MSX1 in Osteogenic Differentiation of Human Dental Pulp Stem Cells
Table 1
Primer and probe sequences used for RT-qPCR.
| Gene | Primer () | Probe |
| MSX1 (sense) | F: CTCGTCAAAGCCGAGAGC | Roche Universal Probe # 7 | R: CGGTTCGTCTTGTGTTTGC | MSX1 (antisense) | F: GCCAGCCCTCTTAGAAACAG | Roche Universal Probe # 50 | R: AATAAAGCAGCCCCTCGTTC | RUNX2 | F: CAGTGACACCATGTCAGCAA | Roche Universal Probe # 66 | R: GCTCACGTCGCTCATTTTG | BMP2 | F: CGGACTGCGGTCTCCTAA | Roche Universal Probe # 49 | R: GGAAGCAGCAACGCTAGAAG | OSX | F: CAGCAGCTAAACTTGGAAGGA | Roche Universal Probe # 76 | R: TGCTTTCGCTTGTCTGAGTC | OCN | F: GCCTCCTGAAAGCCGATGT | 5′-CCAACTCGTCACAGTCCGGATTGAGCT-3′ | R: AAGAGACCCAGGCGCTACCT | ALPL | F: TCACTCTCCGAGATGGTGGT | Roche Universal Probe # 12 | R: GTGCCCGTGGTCAATTCT | SOX9 | F: GTACCCGCACTTGCACAAC | Roche Universal Probe # 61 | R: TCTCGCTCTCGTTCAGAAGTC | PPAR | F: GACAGGAAAGACAACAGACAAATC | Roche Universal Probe # 7 | R: GGGGTGATGTGTTTGAACTTG | SREBP2 | F: GCCCTGGAAGTGACAGAGAG | Roche Universal Probe # 21 | R: TGCTTTCCCAGGGAGTGA | HMGCS1 | F: TCTGTCTACTGCAAAAAGATCCAT | Roche Universal Probe # 59 | R: TGAAGCCAAAATCATTCAAGG | HMGCR | F: GTTCGGTGGCCTCTAGTGAG | Roche Universal Probe # 65 | R: GCATTCGAAAAAGTCTTGACAAC | FDPS | F: GGCCACTCCAGAACAGTACC | Roche Universal Probe # 75 | R: CCTCATATAGCGCCTTCACC | CYP51A1 | F: TGCAGATTTGGATGGAGGTT | Roche Universal Probe # 64 | R: CCTTGATTTCCCGATGAGC | DHCR7 | F: GCCATGGTCAAGGGCTAC | Roche Universal Probe # 60 | R: TTGTAAAAGAAATTGCCTGTGAAT |
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MSX1: msh homeobox 1; RUNX2: runt-related transcription factor-2; BMP2: bone morphogenetic protein-2; OSX: osterix; OCN: osteocalcin; ALPL: alkaline phosphatase liver type; SOX9: SRY- (sex determining region Y-) box 9; PPAR: peroxisome proliferator activated receptor gamma; SREBP2: sterol regulatory element-binding protein 2; HMGCS1: 3-hydroxy-3-methylglutaryl-CoA synthase 1; HMGCR: HMG-CoA reductase; FDPS: farnesyl diphosphate synthase; CYP51A1: Cytochrome P450 Family 51 Subfamily A Polypeptide 1; DHCR7: 7-dehydrocholesterol reductase; F: forward; R: reverse.
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