Induction of Functional Hair-Cell-Like Cells from Mouse Cochlear Multipotent Cells

Figure 6

Voltage-dependent currents elicited from inner ear multipotent-cell-derived FM1-43FX-positive cells. FM1-43FX-positive cells were voltage clamped at −84 mV and stepped between −120 and 50 mV in 10 mV increments. 10 out of 18 cells tested had positive responses and expressed the similar outward and inward K⁺ currents. (a) FM1-43FX-positive cells (green, left) coexpressed the hair cell marker Myosin VIIA (red, center). (b) White light image of a recording electrode on an FM1-43FX-positive cell. (c) Outward K⁺ currents recorded from hair-cell-like cells. (d) Inward K⁺ currents recorded from hair-cell-like cells. (e) Average current-voltage (I-V) curve for the outward and inward K⁺ current measured of hair-cell-like cells at the steady-state level (100–160 ms). (f) Outward K⁺ currents recorded from neonatal mouse cochlea hair cells. (g) Inward K⁺ currents recorded from neonatal mouse cochlea hair cells. (h) Average current-voltage (I-V) curve for the outward and inward K⁺ current of neonatal mouse cochlea hair cells measured at the steady-state level (100–160 ms). (i) Outward K⁺ currents recorded from the cells that are not hair cells. (j) Inward K⁺ currents recorded from the cells that are not hair cells. (k) Average current-voltage (I-V) curve for the outward and inward K⁺ current of the cells that are not hair cells measured at the steady-state level (100–160 ms).

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