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Stem Cells International
Volume 2016, Article ID 8216312, 13 pages
Research Article

Derivation of Pluripotent Cells from Mouse SSCs Seems to Be Age Dependent

1Institute for Anatomy and Cell Biology, Medical Faculty, University of Heidelberg, Im Neuenheimer Feld 307, 69120 Heidelberg, Germany
2Department of Stem Cells and Developmental Biology at the Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, P.O. Box 19395, Tehran 4644, Iran
3Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, P.O. Box 49767, Amol 49767, Iran
4P.O. Box 12 43, 72072 Tübingen, Germany
5Division of Signal Transduction and Growth Control, DKFZ/ZMBH Alliance, Heidelberg, Germany
6Department of Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
7Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran

Received 20 May 2015; Revised 25 July 2015; Accepted 27 July 2015

Academic Editor: Mariusz Z. Ratajczak

Copyright © 2016 Hossein Azizi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

In the supplementary method section, we describe in more detail the material and the methods of embryoid body (EB) formation, neuronal differentiation, cardiomyocyte differentiation, RNA extraction and RT-PCR analysis, gene expression analyses (Fluidigm Biomark), immunfluorescence staining (IMH), electrophysiology, FACS analysis, alkaline phosphatase assay, electron microscopy, production of teratoma and chimeric mice, and the statistical analysis.

  1. Supplementary Material