In Vitro Expansion of Bone Marrow Derived Mesenchymal Stem Cells Alters DNA Double Strand Break Repair of Etoposide Induced DNA Damage

Figure 3

Effects of VP16 on cell cycle distribution and cell cycle inhibitor transcript abundance with passage. (a) Cell cycle distribution of MSCs at various passages exposed to 25 μM VP16 or medium only control for 24 hours. (b) qPCR evaluation of cell cycle inhibitor mRNA expression following exposure of MSCs to 25 μM VP16 for 24 hours followed by 0, 6, or 48 hours of recovery in fresh medium. Values are indicated as fold change relative to untreated control.

(a)

(b)