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Stem Cells International
Volume 2016 (2016), Article ID 8270464, 11 pages
http://dx.doi.org/10.1155/2016/8270464
Research Article

In Vitro Expansion of Bone Marrow Derived Mesenchymal Stem Cells Alters DNA Double Strand Break Repair of Etoposide Induced DNA Damage

1Alexander B. Osborn Hematopoietic Malignancy and Transplantation Program of the West Virginia University Cancer Institute, Robert C. Byrd Health Sciences Center, West Virginia University School of Medicine, Morgantown, WV 26506, USA
2Department of Microbiology, Immunology and Cell Biology, Robert C. Byrd Health Sciences Center, West Virginia University School of Medicine, Morgantown, WV 26506, USA
3Department of Pathology, School of Medicine, West Virginia University, Morgantown, WV 26506, USA

Received 11 August 2015; Revised 22 November 2015; Accepted 25 November 2015

Academic Editor: Silvia Brunelli

Copyright © 2016 Ian Hare et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary materials for this manuscript include two figures and one table. Supplementary Figure 1 contains qPCR data evaluating the transcriptional abundance of pro-apoptotic (A) and anti-apoptotic (B) genes after VP16 exposure with passage. Supplementary Figure 2 shows the average number of Ƴ-H2AX foci (per nucleus) in untreated cells with passage, and Supplementary Table 1 contains all qPCR primer sequences used in this manuscript.

  1. Supplementary Material