Differential Characterization of Two Kinds of Stem Cells Isolated from Rabbit Nucleus Pulposus and Annulus Fibrosus

Table 1

Primers for qRT-PCT analysis.

Gene	Size (bp)	Primers	Type
Collagen I	81	5′-CTG ACT GGA AGA GCG GAG AGT AC-3′	Forward
		5′-CCA TGT CGC AGA AGA CCT TGA-3′	Reverse
Collagen II	84	5′-TGG GTG TTC TAT TTA TTT ATT GTC TTC CT-3′	Forward
		5′-GCG TTG GAC TCA CAC CAG TTA GT-3′	Reverse
Collagen III	255	5′-TTATAAACCAACCTCTTCCT-3′	Forward
		5′-TATTATAGCACCATTGAGAC-3′	Reverse
PPARγ	200	5′-TGG GGA TGT CTC ATA ATG CCA-3′	Forward
		5′-TTC CTG TCA AGA TCG CCC TCG-3′	Reverse
Sox-9	79	5′-AGT ACC CGC ACC TGC ACA AC-3′	Forward
		5′-CGC TTC TCG CTC TCG TTC AG-3′	Reverse
Runx-2	70	5′-TGA TGA CAC TGC CAC CTC TGA-3′	Forward
		5′-GCA CCT GCC TGG CTC TTC T-3′	Reverse
GAPDH	107	5′-ACT TTG TGA AGC TCA TTT CCT GGT A-3′	Forward
		5′-GTG GTT TGA GGG CTC TTA CTC CTT-3′	Reverse