Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity

Figure 1

Generation and characterization of genetically modified ES clones expressing puromycin resistance gene under Pvec. (a) Schematic representation of episomal constructs pPvec and pPvec-EGFP, used for generation of ES clones. (b) The majority of VE-cadherin⁺ cells express EGFP in EBs derived from clones G11 and G8 at d8. (c) Statistical analysis of the percentage of VE-cadherin⁺ cells coexpressing EGFP at d8 from three independent experiments. For each experiment ~600 VE-cadherin⁺ cells were counted. . Scale bar: 20 μm.

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