Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity

Figure 4

Characterization of Pvec⁺ cells. (a) Isl1, GATA4, and VE-cadherin mRNA levels quantification in Pvec⁺ cells in the presence or absence of SB-216763 by real time qPCR. Results in -SB first and -SB second referred to two independent selection/differentiation experiments. (b-c) Isl1⁺ cardiac progenitors express desmin but not cTnT, a marker of differentiated cardiomyocytes. (d) RT-PCR analysis of expanded Pvec⁺ cells showed expression of cardiac, endothelial, and endocardial but not neuroectodermal, endodermal, or pluripotency markers. As positive controls (ctr) mRNA from E14T ESCs differentiation was used. Note the lack of cTnT, MLC2v, and MLC2a expression in expanded Pvec⁺ cells. Scale bar: 20 μm.

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