Research Article
Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity
Figure 6
Characterization of CEDPs-derived products. (a) Statistical analysis of cTnT+ and VE-cadherin+ cells in CEDPs-derived differentiated cells at d12. The total number of cells counted was 10417 in three independent experiments. and . Cells from random fields were photographed (as in representative image left) and counted by Fiji cell-counter. Scale bar: 20 μm. (b) cTnT, Nkx2.5, Isl1, GATA4, VE-cadherin, and Flk1 mRNA levels quantification in CEDPs-derived differentiated cells at d10 by real time qPCR. Results in CEDPs differentiation first and CEDPs differentiation second referred to two independent differentiation experiments.
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