Comparison of Osteogenesis between Adipose-Derived Mesenchymal Stem Cells and Their Sheets on Poly--Caprolactone/-Tricalcium Phosphate Composite Scaffolds in Canine Bone Defects

Table 1

Primers sequences used for quantitative reverse transcription PCR.

Target gene		Primer sequence (5′-3′)
RUNX2	Forward	TGTCATGGCGGGTAACGAT
	Reverse	TCCGGCCCACAAATCTCA
ALP	Forward	TCCGAGATGGTGGAAATAGC
	Reverse	GGGCCAGACCAAAGATAGAG
Osteopontin	Forward	GATGATGGAGACGATGTGGATA
	Reverse	TGGAATGTCAGTGGGAAAATC
Osteocalcin	Forward	CTGGTCCAGCAGATGCAAAG
	Reverse	GGTCAGCCAGCTCGTCACAGTT
BMP7	Forward	TCGTGGAGCATGACAAAGAG
	Reverse	GCTCCCGAATGTAGTCCTTG
AXIN	Forward	ACGGATTCAGGCAGATGAAC
	Reverse	CTCAGTCTGTGCCTGGTCAA
β-catenin	Forward	TACTGAGCCTGCCATCTGTG
	Reverse	ACGCAGAGGTGCATGATTTG
VEGF	Forward	CTATGGCAGGAGGAGAGCAC
	Reverse	GCTGCAGGAAACTCATCTCC
GAPDH	Forward	CATTGCCCTCAATGACCACT
	Reverse	TCCTTGGAGGCCATGTAGAC