Effective Mobilization of Very Small Embryonic-Like Stem Cells and Hematopoietic Stem/Progenitor Cells but Not Endothelial Progenitor Cells by Follicle-Stimulating Hormone Therapy

Figure 1

Representative FACS plots demonstrating gating strategy for HSC and VSELs. First, 2–4 μm size events were gated based on a forward and side scatter (FSC/SSC) dot plot (a). Then the 2–4 μm gate was visualized on sample data using a FSC/SSC dot plot (b). Next, 2–4 μm events were displayed on histogram plot (black peak, FMO control; grey peak identifies positive staining) and Lin⁻CD235a⁻ events were gated (c). Finally, FMO control was used to set the HSC and VSELs gate and exclude the background noise (d). Next, HSCs were defined as Lin⁻CD235a⁻CD45⁺CD133⁺ cells (upper gate) and VSELs were referred to as Lin⁻CD235a⁻CD45⁻CD133⁺ cells (e).