Effective Mobilization of Very Small Embryonic-Like Stem Cells and Hematopoietic Stem/Progenitor Cells but Not Endothelial Progenitor Cells by Follicle-Stimulating Hormone Therapy
Table 2
Detailed characteristics of monoclonal antibodies used in the study.
Name
Clone
Isotype
Format
Additional information
Manufacturer
Mouse anti-human anti-CD34
8G12
IgG1
FITC
This antibody binds to 105–120 kDa single-chain transmembrane glycoprotein, IVD
Becton Dickinson
Anti-human lineage cocktail 2 (lin 2)
FITC
Becton Dickinson
Mouse anti-human anti-CD3
SK7
IgG1
This antibody binds to epsilon chain of the CD3 antigen, IVD
Mouse anti-human anti-CD19
SJ25C1
IgG1
This antibody recognizes a 90 kDa antigen, IVD
Mouse anti-human anti-CD20
L27
IgG1
This antibody binds to phosphoprotein with a molecular weight of 35 or 37 kilodaltons (kDa), depending on the degree of phosphorylation, IVD
Mouse anti-human anti-CD14
MP9
IgG2b
This antibody reacts with a 53–55 kDa glycosylphosphatidylinositol- (GPI-) anchored and single chain glycoprotein, IVD
Mouse anti-human anti-CD56
NCAM16.2
IgG2b
This antibody recognizes a heavily glycosylated 140 kDa isoform of NCAM, a member of the immunoglobulin (Ig) superfamily, IVD
Mouse anti-human anti-CD235a
GA-R2
IgG2b
FITC
This antibody binds to glycophorin A, a sialoglycoprotein present on human red blood cells (RBC) and erythroid precursor cells
Becton Dickinson
Mouse anti-human anti-CD45
HI30
IgG1
PE
This antibody binds to 190, 190, 205, and 220 kDa protein isoforms, RUO
Becton Dickinson
Mouse anti-human anti-CD144
55-7H1
IgG1
PE
This antibody reacts with calcium-independent epitope on cadherin 5, RUO
Becton Dickinson
Mouse anti-human anti-CD309
89106
IgG1
PE
This antibody reacts with CD309 (vascular endothelial growth factor receptor-2 (VEGFR-2))
Becton Dickinson
Mouse anti-human anti-CD133
AC133
IgG1
APC
This antibody reacts with epitope 1 of CD133, RUO
Miltenyi Biotec
IVD: this clone is used for in vitro diagnostics; RUO: suitable for research use only.