Effective Mobilization of Very Small Embryonic-Like Stem Cells and Hematopoietic Stem/Progenitor Cells but Not Endothelial Progenitor Cells by Follicle-Stimulating Hormone Therapy

Table 2

Detailed characteristics of monoclonal antibodies used in the study.

Name	Clone	Isotype	Format	Additional information	Manufacturer
Mouse anti-human anti-CD34	8G12	IgG1	FITC	This antibody binds to 105–120 kDa single-chain transmembrane glycoprotein, IVD	Becton Dickinson
Anti-human lineage cocktail 2 (lin 2)			FITC		Becton Dickinson
Mouse anti-human anti-CD3	SK7	IgG1		This antibody binds to epsilon chain of the CD3 antigen, IVD
Mouse anti-human anti-CD19	SJ25C1	IgG1		This antibody recognizes a 90 kDa antigen, IVD
Mouse anti-human anti-CD20	L27	IgG1		This antibody binds to phosphoprotein with a molecular weight of 35 or 37 kilodaltons (kDa), depending on the degree of phosphorylation, IVD
Mouse anti-human anti-CD14	MP9	IgG2b		This antibody reacts with a 53–55 kDa glycosylphosphatidylinositol- (GPI-) anchored and single chain glycoprotein, IVD
Mouse anti-human anti-CD56	NCAM16.2	IgG2b		This antibody recognizes a heavily glycosylated 140 kDa isoform of NCAM, a member of the immunoglobulin (Ig) superfamily, IVD
Mouse anti-human anti-CD235a	GA-R2	IgG2b	FITC	This antibody binds to glycophorin A, a sialoglycoprotein present on human red blood cells (RBC) and erythroid precursor cells	Becton Dickinson
Mouse anti-human anti-CD45	HI30	IgG1	PE	This antibody binds to 190, 190, 205, and 220 kDa protein isoforms, RUO	Becton Dickinson
Mouse anti-human anti-CD144	55-7H1	IgG1	PE	This antibody reacts with calcium-independent epitope on cadherin 5, RUO	Becton Dickinson
Mouse anti-human anti-CD309	89106	IgG1	PE	This antibody reacts with CD309 (vascular endothelial growth factor receptor-2 (VEGFR-2))	Becton Dickinson
Mouse anti-human anti-CD133	AC133	IgG1	APC	This antibody reacts with epitope 1 of CD133, RUO	Miltenyi Biotec

IVD: this clone is used for in vitro diagnostics; RUO: suitable for research use only.