Evaluation of MSCs and BrdU-labeling efficacy. Primary cultured MSCs exhibit typical elongated, fibroblast-like morphology or large, flattened shape (a). Scale bar 200 μm. MSCs in third passage were positive for BrdU, as immunofluorescence stained with BrdU (b). Scale bar 100 μm. Flow cytometry analysis of the surface markers of bone marrow mononuclear cells: CD29 (99.91%), CD90 (97.52%), CD11b (6.63%), and CD45 (7.07%) (c).