Stem Cells International / 2016 / Article / Fig 3

Research Article

Restoration of a Critical Mandibular Bone Defect Using Human Alveolar Bone-Derived Stem Cells and Porous Nano-HA/Collagen/PLA Scaffold

Figure 3

Proliferation and osteogenic differentiation potential of aBMSCs on nHAC/PLA. (a) SEM analysis showed that nHAC/PLA was similar to natural bone in terms of microstructure. (b) After 3 days of culture, the aBMSCs adhered to, extended, and connected with each other and produced ECM on the nHAC/PLA surface. The aBMSCs (c–f) were spindle, triangle, or cube shaped, with developed cytoplasmic extensions attached to the scaffold. (g) The MTS results demonstrated that the aBMSCs were proliferative on days 1, 3, 5, and 7 after seeding onto nHAC/PLA (, mean ± SD). (h) Real-time PCR revealed that osteogenic induction for 7 days promoted aBMSC expression of OCN, Runx2, and Osterix (, mean ± SD).Compared with nHAC/PLA + aBMSCs. Differences are significant at . Magnification: (a, b) ×100 SE; (c) ×500 SE; (d) ×1.50 k SE; (e) ×5.00 k SE; (f) ×10.0 k SE.
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