Bone Formation from Porcine Dental Germ Stem Cells on Surface Modified Polybutylene Succinate Scaffolds

Figure 9

Confocal microscopy images of cells seeded on (a, b) unmodified; (c, d) fibronectin modified; and (e, f) laminin modified PBS scaffolds; (g, h) TCP after (a, c, e, g) 10 days and (b, d, f, h) 20 days of incubation. Green stains show the synthesis of collagen in the cytoskeleton of the cells stained with collagen type I and blue stains show the nucleus of the cells stained with TO-PRO-3 Iodide. Scale bars (a, b, c, d, e, f) 194 μm, (g) 85 μm, and (h) 41 μm.

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