Innate Immune Response of Human Embryonic Stem Cell-Derived Fibroblasts and Mesenchymal Stem Cells to Periodontopathogens

<div>Differentiation of hESCs to hESC-MSCs. (a–d) The photomicrographs demonstrate the differentiation of hESCs to hESC-MSCs through embryoid bodies and embryoid body outgrowth under low-glucose differentiation conditions. (e) Characterization of hESC-MSCs by real-time RT-PCR for pluripotency (OCT4, SOX2, and NANOG) and mesenchymal (<i>COL1A1</i>,<i> COL3A1</i>) and osteogenic (<i>RUNX2</i>,<i> OSTERIX</i>, and<i> OPG</i>) lineage associated transcripts. (f) Flow cytometry characterization of hESC-MSCs for expression of surface markers. Scale bar in (a) and (c): 500 <i>µ</i>m. Scale bar in (b) and (d): 200 <i>µ</i>m.</div>

Stem Cells International

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Figure 2

Figure 2: Innate Immune Response of Human Embryonic Stem Cell-Derived Fibroblasts and Mesenchymal Stem Cells to Periodontopathogens 