Research Article

Innate Immune Response of Human Embryonic Stem Cell-Derived Fibroblasts and Mesenchymal Stem Cells to Periodontopathogens

Table 1

Primer sequences used for real-time RT-PCR.

GeneDescriptionPrimer sequenceProduct length

β-ACTINActin, betaF: CCAAGGCCAACCGCGAGAAGATGAC
R: AGGGTACATGGTGGTGCCGCCAGAC
587 bp

OCT4Octamer-binding transcription factor 4; POU class 5 homeobox (POU5F1)F: CGTGAAGCTGGAGAAGGAGAAGCTG
R: AAGGGCCGCAGCTTACACATGTTC
247 bp

SOX2SRY- (sex determining region Y-) Box F: CCGCATGTACAACATGATGG
R: CTTCTTCATGAGCGTCTTGG
370 bp

P4HβProlyl- 4-hydroxylase, beta subunitF: GTCTTTGTGGAGTTCTATGCCC
R: GTCATCGTCTTCCTCCATGTCT
338 bp

COL1A1Collagen type I, alpha-1F: GAACGCGTGTCAATCCCTTGT
R: GAACGAGGTAGTCTTTCAGCAACA
91 bp

COL3A1Collagen type III, alpha-1F: AACACGCAAGGCTGTGAGACT
R: GCCAACGTCCACACCAAATT
88 bp

RUNX2Runt-related transcription factor 2F: TGAGAGCCGCTTCTCCAACC
R: GCGGAAGCATTCTGGAAGGA
266 bp

IL-1βInterleukin-1-betaF: AAGCTGAGGAAGATGCTG
R: ATCTACACTCTCCAGCTG
390 bp

IL-6Interleukin-6 (interferon, beta 2)F: TGCGTCCGTAGTTTCCTTCT
R: GCCTCAGACATCTCCAGTCC
141 bp

IL-8Interleukin-8F: GGTGCAGTTTTGCCAAGGAG
R: TTCCTTGGGGTCCAGACAGA
183 bp

OPGOsteoprotegerinF: GCCTGGCACCAAAGTAAACG
R: TACGAAGCTGCTCGAAGGTG
209 bp

OsterixOsterix, transcription factor Sp7F: CTCTGGAGTCAGAGTAGGACTGT
R: CAAGGAGCCAGGCAGATGGA
197 bp

TLR-2Toll-like receptor-2F: GCCTCTCCAAGGAAGAATCC
R: TCCTGTTGTTGGACAGGTCA
144 bp

TLR-4Toll-like receptor-4F: GGCAGCTCTTGGTGGAAGTT
R: ACAAGCACACTGAGGACCGA
136 bp

NANOGF: TGATTTGTGGGCCTGAAGAAAA
R: GAGGCATCTCAGCAGAAGACA
60 bp

VIMENTINF: AGTCCACTGAGTACCGGAGAC
R: CATTTCACGCATCTGGCGTTC
98 bp