Stromal Derived Factor-1/CXCR4 Axis Involved in Bone Marrow Mesenchymal Stem Cells Recruitment to Injured Liver

Figure 3

Injured liver lysates induce BMSCs migration in vitro. (a) Changes of serum ALT and AST level after TAA injection; (b) HE stained liver tissue sections after TAA injection; (c) the concentrations of SDF-1 of liver lysates detected by ELISA at different time point of TAA induced liver injury; (d) the migrated cells induced by normal liver lysates and TAA injured liver lysates. More cell migration was induced by TAA injured liver lysates which could be partially blocked by AMD3100 (24 μg/mL) or anti-CXCR4 antibody (20 μg/mL). The migrated cells were observed under fluorescence microscope and the graph indicated the number of migrated cells.

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