Differentiation and Molecular Properties of Mesenchymal Stem Cells Derived from Murine Induced Pluripotent Stem Cells Derived on Gelatin or Collagen

Figure 2

Effects of gelatin or collagen on germ-layer associated genes expression in iMSCs. Time-course (day 8 to day 39) genes expression in each cell with semiquantitative reverse transcription polymerase chain reaction. iPS medium; iPSCs on gelatin (Gel) or collagen (Col), control medium; iPSCs in 8 day-derivation without dexamethasone and ascorbic acid, derivation medium; iPSCs in 8 day-derivation, expansion medium; iMSCs at passages 2 (P2) and P7 in expansion medium, BMMSCs; bone marrow MSC at P5, MSC; MSCs from Invitrogen, and mouse embryonic fibroblast (MEF).