Three-Dimensional Aggregates Enhance the Therapeutic Effects of Adipose Mesenchymal Stem Cells for Ischemia-Reperfusion Induced Kidney Injury in Rats

Figure 2

Characters of 3D AdMSCs aggregates. (a) Viability of AdMSCs cultured in 2D monolayer. (b) Viability of 3D AdMSCs aggregates 1 d, 3 d, and 7 d after seeding in microwell. Live or dead cells were stained with FDA (live, green) PI (dead, red). (c) Fluorescent images of 2D AdMSCs or 3D aggregates stained with fibronectin and laminin. (d) mRNA expression of fibronectin and laminin in 2D AdMSCs or 3D aggregates. compared with 2D AdMSCs.

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