Expression of the Wnt Receptor Frizzled-4 in the Human Enteric Nervous System of Infants
Table 2
Primary antibodies used for cell analysis.
Primary antibodies
Species
Dilution
Manufacturer
α-smooth muscle actin
Rabbit
1 : 100
Spring Bioscience, Pleasanton, USA
c-kit
Rabbit
1 : 500
Dako, Hamburg, Germany
Fzd4 (clone CH3A4)
Mouse
Undiluted
In-house (HJB)
Fzd4 PE (clone CH3A4)
Mouse
1 : 50
Biolegend, San Diego, USA
GFAP
Rabbit
1 : 400
Dako, Hamburg, Germany
Nestin
Rabbit
1 : 1000
Abcam, Cambridge, UK
p75NTR
Rabbit
1 : 500
Promega, Madison, USA
p75NTR APC
Rabbit
1 : 50
Miltenyi, Bergisch Gladbach, Germany
Peripherin
Rabbit
1 : 200
Merck Millipore, Darmstadt, Germany
Monoclonal mouse anti-human antibody CH3A4 against frizzled-4 was raised by immunization with the retinoblastoma cell line WERI-RB-1 and specificity for frizzled-4 was verified by the selective recognition of HEK-293 cells transfected with human frizzled-4. This molecule was clustered to CD344 at the HCDM workshop in Quebec, Canada (http://www.hcdm.org/).