Vitamin D Effects on Osteoblastic Differentiation of Mesenchymal Stem Cells from Dental Tissues

Figure 1

ALP positivity and mineralized nodules formation. (a) ALP histochemical assay (purple staining) performed on DBSCs seeded and differentiated for 7 days with vehicle (−) and 1,25(OH)₂D₃ (+). The graph shows quantification of positive staining as percentage compared to untreated cells (−) () and is representative for 3 independent donors. Data are presented as mean ± SEM. Student’s -test was used for single comparisons. (b) Alizarin Red (red staining) shows mineralized nodules formation by DBSCs incubated for 21 days in osteogenic medium with vehicle (−) and 1,25(OH)₂D₃ (+). The graph shows quantification of ARS with the Optical Density (OD) at 405 nm (b); indicates statistical significance (). Data are representative for 3 independent donors and are presented as mean ± SEM. Student’s -test was used for single comparisons.

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