Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton’s Jelly-Derived Mesenchymal Stem Cells
Morphology and expression of cardiac-specific genes and protein in WJ MSCs (Wharton’s jelly-derived mesenchymal stem cells) in the early and middle phases after cardiac induction. (a-b) After being treated with 5-Aza for 14 days, cardiomyocyte-like cells with a stick-like morphology were found in induced P7 and P14 cells. Bar: 100 μm. (c) The gene expression of GATA4 and Nkx2.5 was upregulated after cardiac induction. (d) No α-actinin protein expression was detected in normal P7 and P14 WJ MSCs (P7 control and P14 control). However, α-actinin protein expression appeared in the induced cells (induced P7 and induced P14). (e) Semiquantified analysis of western blot results showed that α-actinin protein expression in the group of induced P7 was significantly higher than induced P14 (), which indicated that serial passage significantly decreased the cardiac differentiation capability of WJ MSCs.
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.