Effect of Fibroblast Growth Factor 2 on Equine Synovial Fluid Chondroprogenitor Expansion and Chondrogenesis

Table 1

Primers used in the qPCR reactions.

Gene; (amplicon size)		Primers	Annealing temperature
EF1-alpha; (328 bp)	S	5′ CCCGGACACAGAGACTTCAT	62.1°C
	A	5′ AGCATGTTGTCACCATTCCA
Col II; (223 bp)	S	5′ AGCAGGAATTTGGTGTGGAC	62.1°C
	A	5′ TCTGCCCAGTTCAGGTCTCT
Col X; (244 bp)	S	5′ TGCCAACCAGGGTGTAACAG	62.1°C
	A	5′ ACATTACTGGGGTGCCGTTC
ALP; (260 bp)	S	5′ CCACGTCTTCACATTTGGTG	54.2°C
	A	5′ AGACTGCGCCTGGTAGTT
Aggrecan; (202 bp)	S	5′ GACGCCGAGAGCAGGTGT	62.1°C
	A	5′ AAGAAGTTGTCGGGCTGGTT
Sox9; (304 bp)	S	5′ GAACGCACATCAAGACGGAG	56.2°C
	A	5′ CTGGTGGTCTGTGTAGTCGT
Mef2c; (55 bp)	S	5′ CCCAACTTTGAGTGCCAGT	55.3°C
	A	5′ ATGTGAGGTCTCCACCCATC
Runx2; (115 bp)	S	5′ CAGACCAGCAGCACTCCATA	56.8°C
	A	5′ GAGCGTCAACACCATTC