Polydatin Protects Bone Marrow Stem Cells against Oxidative Injury: Involvement of Nrf 2/ARE Pathways

Figure 2

Effects of polydatin on the cell viability of BMSCs exposed to H₂O₂. Cells were pretreated with different concentrations of polydatin for 2 h followed with H₂O₂ (600 μM) for 24 h. (a) Structure of polydatin. (b) Cells were treated with different concentrations of H₂O₂ for 24 h. (c, d) Cell viability was measured by MTT assay and cells were photographed under phase-contrast optics. (A) CT, (B) H₂O₂, (C) polydatin 3 μM + H₂O₂, (D) polydatin 10 μM + H₂O₂, and (E) polydatin 30 μM + H₂O₂. (e) Cell death was measured by LDH assay. Bar graph represents independent experiments, each performed in triplicate. One-way ANOVA followed by Tukey’s test. Data are presented as means ± S.D. and versus control group. versus H₂O₂-treated group.

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