BMP4 Cooperates with Retinoic Acid to Induce the Expression of Differentiation Markers in Cultured Mouse Spermatogonia

Figure 4

BMP4 protein is mainly expressed in mouse germ cells. (a) IFA analysed the localization of BMP4 and DAZL in 7-day mouse testis section (arrow head). (b) In adult mouse testis section, BMP4 was predominantly expressed in spermatocytes (arrow) and round spermatids (arrow head). (c-d) In testicular cells isolated from 4-5-day mice, BMP4 was predominantly expressed in germ cells (arrow) and, to a less extent, in Sertoli cells (arrow head). (e-f) BMP4 was also expressed in P3 (e) and P33 (f) cultured spermatogonia. Scale bar: 20 μm.

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