BMP4 Cooperates with Retinoic Acid to Induce the Expression of Differentiation Markers in Cultured Mouse Spermatogonia

Table 1

PCR primer sequences and product size.

Gene	Forward primer (5′–3′)	Reverse primer (5′–3′)	Product size
BMP4	TCGTTACCTCAAGGGAGTGG	GGCGACGGCAGTTCTTATTC	159 bp
BMPR1A	GGGTCGTTACAACCGTGAT	CAACCTGCCGAACCATCT	164 bp
BMPR1B	TCAATGTCGTGACACTCCCATTCCT	TGCTGTACCGAGGTCGGGCT	245 bp
ActRIIB	CGAGCGCTTCACCCACTTG	CACCACGACACCACGGCAC	650 bp
ActRII	CCGGAGATGGAAGTCACA	CACATCCACACTGGTGCC	432 bp
BMPRII	AGGCCCAATTCTCTGGATCT	CACTGCCATTGTTGTTGACC	207 bp
Actin	CAGCCTTCCTTCTTGGGTAT	TGGCATAGAGGTCTTTACGG	100 bp
Stra8	ACAAGAGTGAGGCCCAGCAT	CCTCTGGATTTTCTGAGTTGCA	71 bp
c-Kit	GCCACGTCTCAGCCATCTG	GTCGGGATCAATGCACGTCA	119 bp