Comparison of Immunomodulation Properties of Porcine Mesenchymal Stromal/Stem Cells Derived from the Bone Marrow, Adipose Tissue, and Dermal Skin Tissue

Figure 1

Analysis of alkaline phosphatase (AP) and CD marks in mesenchymal stromal/stem cells (MSCs). For analysis of AP activity, each type of passage 1 MSCs was cultured until ca. 80% confluence on 35 mm dishes, fixed in 3.7% formalin, and stained with a BCIP/NBT kit (A). a, b, and c show A-MSCs, BM-MSCs, and DS-MSCs, respectively, derived from a female micropig. MSCs were observed by both macrography (a–c) after AP staining and micrography before (a-1, b-1, and c-1; scale bars = 500 μm) and after (a-2, b-2, and c-2; scale bars = 200 μm) AP staining. The presence of purple/brown color on AP staining was judged to be a positive reaction. (B) MSC-positive (CD90, 105, and 29) and -negative (CD45) CD marks measured in cells per sample by flow cytometry. Open black histogram represents the isotype-matched control, and green and red open histograms represent positive CD marks.