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Stem Cells International
Volume 2016, Article ID 9586751, 13 pages
http://dx.doi.org/10.1155/2016/9586751
Research Article

Differentiation-Associated MicroRNA Alterations in Mouse Heart-Derived Sca-1+CD31 and Sca-1+CD31+ Cells

Qiong Wu,1,2,3 Jinxi Zhan,1,2,3 Yun Li,1,2,3 Xiaoxia Wang,1,2,3 Lu Xu,4 Juan Yu,1,2,3 Shiming Pu,1,2,3 and Zuping Zhou1,2,3

1School of Life Sciences, Guangxi Normal University, Guilin 541004, China
2Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical Technology, Guangxi Normal University, Guilin 541004, China
3Research Center for Biomedical Sciences, Guangxi Normal University, Guilin 541004, China
4Jilin Medical College, Jilin 132013, China

Received 22 November 2015; Revised 2 March 2016; Accepted 20 March 2016

Academic Editor: Tobias Cantz

Copyright © 2016 Qiong Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cardiac resident stem/progenitor cells (CSC/CPCs) are critical to the cellular and functional integrity of the heart because they maintain myocardial cell homeostasis. Several populations of CSC/CPCs have been identified based on expression of different stem cell-associated antigens. Sca-1+ cells in the cardiac tissue may be the most common CSC/CPCs. However, they are a heterogeneous cell population and, in transplants, clinicians might transplant more endothelial cells, cardiomyocytes, or other cells than stem cells. The purposes of this study were to (1) isolate CSC/CPCs with LinCD45Sca-1+CD31 and LinCD45Sca-1+CD31+ surface antigens using flow-activated cell sorting; (2) investigate their differentiation potential; and (3) determine the molecular basis for differences in stemness characteristics between cell subtypes. The results indicated that mouse heart-derived Sca-1+CD31 cells were multipotent and retained the ability to differentiate into different cardiac cell lineages, but Sca-1+CD31+ cells did not. Integrated analysis of microRNA and mRNA expression indicated that 20 microRNAs and 49 mRNAs were inversely associated with Sca-1+CD31 and Sca-1+CD31+ subtype stemness characteristics. In particular, mmu-miR-322-5p had more targeted and inversely associated genes and transcription factors and might have higher potential for CSC/CPCs differentiation.