Differentiation-Associated MicroRNA Alterations in Mouse Heart-Derived Sca-1⁺CD31⁻ and Sca-1⁺CD31⁺ Cells

Figure 1

Analysis of CD31 and Sca-1 expression by IF staining. (a–c) Sca-1 expression in Sca-1⁺CD31⁻ cells with Sca-1 only in Sca-1⁺CD31⁻ cells. (d) Sca-1⁺CD31⁻ cell morphology using inverted microscopy and ordinary light. (e–h) Coexpression of CD31 and Sca-1 in Sca-1⁺CD31⁺ cells. (i) Sca-1⁺CD31⁺ cell morphology as above. Merging fluorescent signals showed no heterogeneous populations after FACS-based sorting. All cells were triple-stained for Sca-1 (red), CD31 (green), and DAPI (blue). Scale bars, 10 μm.