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Figure 2: Differentiation potential of Sca-1+CD31 cells into cardiomyocyte, smooth muscle, and endothelial cells in vitro using FACS. Cells were stained for specific markers after induction. Immunofluorescence staining (green) of cardiac-specific marker-Cardiac Troponin I (c), smooth muscle marker α-Smooth Muscle Actin (g), endothelial cell marker-Von Willebrand Factor (k). (a, e, i) Controls with bright light to show cell morphology after induction. (b, f, j) Nuclei (blue) counterstained with DAPI. (d, h, l) Merged images (b-c), (f-g), and (j-k). Scale bars, 50 μm.