Dasatinib and Doxorubicin Treatment of Sarcoma Initiating Cells: A Possible New Treatment Strategy

Figure 3

Cell viability was determined by non-Radioactive Cell Proliferation Assay (MTS) for erlotinib (concentrations: 0.01–5 μM), afatinib (concentrations: 0.01–5 μM), and dasatinib (concentrations: 0.01–5 μM) in hMSC-TERT4 (solid line) and hMSC-TERT20-CE8 (dashed lines). The results are presented as fold changes compared to the nontreated cells. The MTS assays were performed twice with total of 9 replicates for the erlotinib treated cells. The MTS assay was performed once with 6 replicates for the afatinib treated cells. The MTS assays were performed two or three times with at least a total of 12 replicates for the dasatinib treated cells. The cell viability is presented as mean value with 95% confidence interval for direct comparison. The cells were treated for 72 hours.

(a)

(b)

(c)