Dasatinib and Doxorubicin Treatment of Sarcoma Initiating Cells: A Possible New Treatment Strategy

Figure 4

Cell viability expressed as mean fold changes and 95% confidence interval after treatment of hMSC-TERT20-CE8 which are clonal cells lines derived from stromal stem cell line and with the ability to form sarcoma-like tumours in mice. (1) Control, no treatment. (2) Doxorubicin 25 nM. (3) Doxorubicin 25 nM + dasatinib 5 μM. (4) Doxorubicin 25 nM + erlotinib 5 μM. (5) Doxorubicin 50 nM. (6) Doxorubicin 50 nM + dasatinib 5 μM. (7) Doxorubicin 50 nM + erlotinib 5 μM. (8) Doxorubicin 50 nM + afatinib 5 μM. (9) Dasatinib 5 μM. (10) Erlotinib 5 μM. (11) Afatinib 5 μM. The experiments were performed twice with at least a total of 6 replicates of each cell line. Significant results compared to the nontreated cells are marked with whereas significant results compared to the corresponding doxorubicin treatment are marked with nontreatment .